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A new Eco RI family of satellite DNA in lampreys
Author(s) -
Boán Francisco,
Viñas Ana,
Rodríguez Jose Manuel,
Sánchez Laura,
Gómez-Márquez Jaime
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00947-7
Subject(s) - lamprey , ecori , satellite dna , satellite , petromyzon , biology , dna , evolutionary biology , genetics , gene , paleontology , genome , restriction enzyme , astronomy , physics
Satellite DNA sequences have been studied in several groups of organisms. However, until now this type of sequence has not been characterized in cyclostomata, an evolutionarily important class of vertebrates. In the present work, we report the molecular characterization of a new family of satellite DNA in lampreys ( Petromyzon marinus ). Digestion of lamprey DNA with Eco RI identified a series of very abundant AT‐rich (60% A+T) repeating units, with short stretches of AT, that are multimers of 370 bp. Southern blot analysis and comparison with the satellite DNA sequences deposited in the databases indicate that this new family of satellite DNA is exclusive to lampreys. The distribution of this Eco RI satellite DNA on lamprey chromosomes was analyzed by in situ hybridization. The evolutionary origin of this satellite is briefly discussed.