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Excess formation of lysophosphatidic acid with age inhibits myristic acid‐induced superoxide anion generation in intact human neutrophils
Author(s) -
Ito Yoshikazu,
Ponnappan Usha,
Lipschitz David A.
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00937-4
Subject(s) - superoxide , myristic acid , lysophosphatidic acid , chemistry , endocrinology , medicine , biochemistry , microbiology and biotechnology , biology , enzyme , receptor , fatty acid , palmitic acid
A superoxide anion generation rate upon exposure to myristate of 1.93 ± 0.34 nmol/min/10 6 cells in neutrophils from elderly human donors was significantly less than a value of 3.02 ± 0.48 nmol/min/10 6 neutrophils from young donors. Myristate activation resulted in equal increases of AA in both the young and the old indicating no effect of aging on the PLA 2 pathway to response. By contrast, the PLD‐induced generation of PA was significantly higher in the old than in the young. In addition, myristate induced a significant age‐related enhancement in LPA generation, in the old but not in the young. The mass of LPA generated following activation was 3.5 nmol/ 2.5 × 10 7 cells/ml in the young while in the old it averaged 7.0 nmol/2.5 × 10 7 cells/ml. The inhibitory effects of LPA may explain the age‐related impaired ability to generate superoxide anion following activation by myristate.