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SNAP‐25 is present on chromaffin granules and acts as a SNAP receptor
Author(s) -
Tagaya Mitsuo,
Genma Tomohiro,
Yamamoto Akitsugu,
Kozaki Shunji,
Mizushima Shoji
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00932-5
Subject(s) - snap , chemistry , receptor , biophysics , microbiology and biotechnology , biology , biochemistry , computer science , computer graphics (images)
SNAP‐25 is located on the plasma membrane and essential for exocytosis of neurotransmitters. It was suggested that SNAP‐25 and syntaxin 1 via the interaction with VAMP‐2 located on synaptic vesicles mediate the docking of the vesicles with the plasma membrane. In the present study, by means of biochemical and morphological analyses, we showed that SNAP‐25 is present on chromaffin granules as well as on the plasma membrane. Reconstitution and immunoprecipitation analyses revealed that SNAP‐25 on chromaffin granules has essentially the same properties as does SNAP‐25 on the plasma membrane.