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The liver‐specific promoter of the human insulin‐like growth factor‐II gene contains two negative regulatory elements
Author(s) -
Rodenburg Richard J.T.,
Krijger Jorrit-Jan T.,
Holthuizen P.Elly,
Sussenbach John S.
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00926-x
Subject(s) - enhancer , gene , promoter , biology , growth factor , nucleotide , microbiology and biotechnology , promoter activity , transcription factor , chemistry , genetics , gene expression , receptor
The adult liver‐specific IGF‐II promoter P1 is activated by CCAAT/Enhancer Binding Proteins α and β. Here we present evidence that promoter P1, in addition to positively regulating elements, contains two elements of 67 nucleotides that form an inverted repeat (IR) and suppress P1 activity. The two IR elements are specifically bound by a protein (inverted repeat binding factor, IRBF). The amounts of IRBF in various cell lines correlate with the levels of suppression of P1 activity, suggesting that this factor is responsible for the suppression of P1 mediated by the IR elements.