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Two phosphatidylethanol classes separated by thin layer chromatography are produced by phospholipase D in rat brain hippocampal slices
Author(s) -
Sarri Elisabet,
Servitja Joan-Marc,
Picatoste Fernando,
Claro Enrique
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00906-4
Subject(s) - phosphatidylethanol , phosphatidylcholine , phospholipase d , thin layer chromatography , chemistry , chromatography , arachidonic acid , phospholipase , phospholipase a2 , biochemistry , enzyme , phospholipid , membrane
Noradrenaline‐ and ionomycin‐stimulated as well as basal phospholipase D activity from rat hippocampus produced, in the presence of ethanol, two different classes of [ 32 P]phosphatidylethanol (designated I and II), which were separated by thin layer chromatography. Endogenous labeling experiments using 3 H‐fatty acids showed that two different classes of phosphatidylcholine, separated by two‐dimensional TLC, one enriched with high incorporation of [ 3 H]arachidonic acid (B) and the other with [ 3 H]myristic acid (A), were the most likely sources for the two classes of phosphatidylethanol. Experiments where individual 32 P‐phospholipids extracted from [ 32 P]Pi‐labeled hippocampal slices were incubated with cabbage phospholipase D, in the presence of ethanol, showed that each class of [ 32 P]phosphatidylcholine, i.e. A and B, produced a different band of [ 32 P]phosphatidylethanol, with the same mobility in TLC as phosphatidylethanol II and I, respectively.