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A novel gene codes for a putative G protein‐coupled receptor with an abundant expression in brain
Author(s) -
O'Dowd Brian F.,
Nguyen Tuan,
Lynch Kevin R.,
Kolakowski Lee F.,
Thompson Miles,
Cheng Regina,
Marchese Adriano,
Ng Gordon,
Heng Henry H.Q.,
George Susan R.
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00901-5
Subject(s) - 5 ht5a receptor , biology , gene , gabbr1 , complementary dna , microbiology and biotechnology , genbank , gene expression , northern blot , southern blot , genetics , protease activated receptor 2
Following the cloning of the dopamine receptors we continued a search of the human genome for related genes. We searched an EST data base and discovered cDNA fragments encoding novel G protein‐coupled receptor genes. The available GenBank sequence of one of these EST fragments showed that it encoded a receptor with closest similarity to the D2 dopamine and adrenergic receptors. This cDNA was used to isolate the gene (GPR19), and the encoded receptor also demonstrated similarity with the neuropeptide Y receptor. The gene was mapped to chromosome 12, in region p13.2–12.3. Northern blot analysis revealed expression of GPR19 in peripheral regions, and brain regions significantly overlapping with the D2 receptor gene expression. A sequence of the rat orthologue of GPR19 was obtained and in situ hybridization analysis demonstrated a very abundant expression in rat brain.