Premium
Assignment of protein disulphides by a computer method using mass spectrometric data
Author(s) -
Caporale Carlo,
Sepe Ciro,
Caruso Carla,
Pucci Piero,
Buonocore Vincenzo
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00894-0
Subject(s) - edman degradation , peptide , chemistry , carboxypeptidase , combinatorial chemistry , fragment (logic) , sequence (biology) , molecular mass , mass spectrometry , peptide sequence , chromatography , algorithm , biochemistry , computer science , enzyme , gene
We designed a computer program for the assignment of protein disulphides using mass spectrometric data. All the theoretical linear peptides containing from one to three cysteines are generated on the basis of the protein sequence. Their combination ways are determined in order to create all the possible disulphide‐bridged fragments containing from two to six cysteines and to calculate their molecular weight. One, two and three S‐S bridges per linked fragment were considered, taking into account the possibility that the fragments contain unabridged residues. The mass data obtained from the spectral analysis of peptide mixtures of the digested protein are then associated to the fitting structures of disulphide‐bridged peptides, giving rise to the primary output. This output can then be screened by using information on the specificity of the proteolytic agent(s) used and/or any further mass data provided by Edman degradation and/or carboxypeptidase treatment of the peptide mixtures. The need for such a computer aid is discussed and examples of application are given.