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Evidence for the presence of kinetin in DNA and cell extracts
Author(s) -
Barciszewski Jan,
Siboska Gunhild E.,
Pedersen Bent O.,
Clark Brian F.C.,
Rattan Suresh I.S.
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00884-8
Subject(s) - dna , deoxyribose , chemistry , kinetin , moiety , biochemistry , dna damage , stereochemistry , in vitro , tissue culture
In contrast to the current view that kinetin ( N 6 ‐furfuryladenine) is an unnatural and synthetic compound, we have detected it in commercially available DNA, in freshly extracted cellular DNA from human cells and in plant cell extracts by two independent methods. First, we discovered that N 6 ‐furfuryladenine has electrochemical properties that can be applied for monitoring this modified based by a HPLC/UV/EC method. Second, we have confirmed electrochemical assignments by mass‐spectrometric analysis. A pathway of kinetin formation is proposed in which the formation of furfural by oxidative damage of the deoxyribose moiety of DNA is followed by its reaction with adenine residues to form N 6 ‐furfuryladenine. Since this modification can lead to mutations, the odd DNA base has to be removed by repair enzymes.