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Molecular cloning and tissue‐specific expression of mouse kidney 6‐phosphofructo‐2‐kinase/fructose‐2,6‐bisphosphatase
Author(s) -
Batra Ritu Sonia,
Brown Ruth,
Brown Garry K.,
Craig Ian W.
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00878-2
Subject(s) - complementary dna , microbiology and biotechnology , phosphofructokinase 2 , northern blot , biology , exon , alternative splicing , gene , cdna library , molecular cloning , isozyme , homology (biology) , gene expression , cloning (programming) , sequence analysis , southern blot , messenger rna , fructose 1,6 bisphosphatase , nucleic acid sequence , biochemistry , enzyme , computer science , programming language
A 1932 bp cDNA clone encoding a novel isozyme of 6‐phosphofructo‐2‐kinase/fructose‐2,6‐bisphosphatase (PFK‐2/FBPase‐2) was isolated from a mouse kidney cDNA library. The sequence encodes 519 amino acids and, based on homology to rat heart genomic sequence, appears to be the product of alternative splicing from PFK‐2/FBPase‐2 gene B with an extended version of exon 15. Northern blot analysis indicated that this clone corresponds to an 8 kb mRNA expressed in multiple tissues, with the strongest signal in kidney, and detects several additional transcripts which may be alternatively spliced from gene B.