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Microtubule‐active drugs suppress the closure of the permeability transition pore in tumour mitochondria
Author(s) -
Evtodienko Yuri V.,
Teplova Vera V.,
Sidash Serguei S.,
Ichas François,
Mazat Jean-Pierre
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00875-7
Subject(s) - microtubule , colchicine , tubulin , mitochondrial permeability transition pore , mitochondrion , cytoskeleton , microbiology and biotechnology , chemistry , oxidative phosphorylation , membrane permeability , phosphorylation , biophysics , biology , apoptosis , biochemistry , membrane , programmed cell death , cell , genetics
We report the effects of anticancer drugs, inhibitors of microtubule organisation, on the mitochondrial permeability transition pore (PTP) in Ehrlich ascites tumour cells. Taxol (5–20 μM) and colchicine (100–500 μM) prevented closing of the cyclosporin A‐sensitive PTP. No taxol or colchicine effects on oxidative phosphorylation were observed in the range of concentrations used. We suggest that either membrane‐bound tubulin per se can be part of PTP and/or the attachment of mitochondria to the microtubular network is essential for PTP regulation. The taxol inhibition of PTP closure, mediated through interaction with the cytoskeleton, sheds new light on the cytotoxic properties of this anticancer drug.