Premium
Alzheimer's presenilin 1 gene expression in platelets and megakaryocytes
Author(s) -
Vidal Ruben,
Ghiso Jorge,
Wisniewski Thomas,
Frangione Blas
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00845-9
Subject(s) - presenilin , gene , exon , biology , western blot , platelet , rna splicing , microbiology and biotechnology , coding region , gene expression , genetics , alzheimer's disease , immunology , disease , medicine , pathology , rna
The presenilin 1 (PSI) gene located on chromosome 14 has been linked with the majority of early‐onset FAD. The normal biological role of PS1 as well as the mechanism by which mutations in PS1 cause FAD remains unknown. PS1 expression in platelets and the Dami megakaryocytic cell line was examined by Western blot analysis and RT‐PCR. Using an anti‐N‐terminus PS1 antibody we detected PS1 immunoreactive bands of 44, 32 and 27 kDa in both cell types. After RT‐PCR we observed that platelets and megakaryocytes carry at least four different PS1 transcripts. One of them is a novel PS1 splice variant that lacks the coding sequence for exon 10 resulting in a shorter 409 amino acid protein.