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Stimulation of tryptophan hydroxylase production in a serotonin‐producing cell line (RBL2H3) by intracellular calcium mobilizing reagents
Author(s) -
Hasegawa Hiroyuki,
Kojima Masayo,
Iida Yoshiko,
Oguro Kazuya,
Nakanishi Nobuo
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00834-4
Subject(s) - tryptophan hydroxylase , tryptophan , stimulation , serotonin , enzyme , calcium in biology , intracellular , chemistry , calcium , biology , biochemistry , medicine , endocrinology , amino acid , receptor , serotonergic , organic chemistry
RBL2H3 cells showed a remarkable increase in their level of tryptophan hydroxylase (up to 25‐fold), the rate‐limiting enzyme in serotonin biosynthesis, by stimulation with intracellular calcium mobilizers A23187, thapsigargin, and t BuBHQ as well as by stimulation with an antigen in the presence of IgE. The increase in the enzyme protein was visualized by Western blot analysis using anti‐tryptophan hydroxylase antiserum. The enzyme turnover (Hasegawa et al., FEBS Lett ., 368 (1995) 151–154) was not showed down during the rise in tryptophan hydroxylase. Actinomycin D prevented the stimulation‐induced elevation of the enzyme. These findings strongly suggest that this stimulation was achieved by the accelerated biosynthesis of tryptophan hydroxylase.