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Immunohistochemical and immunoblotting identification of protein phosphatase 1γ1 in rat salivary glands
Author(s) -
Shirakawa Satoko,
Mochizuki Hajime,
Kobayashi Shigeru,
Takehara Tadamichi,
Shima Hiroshi,
Nagao Minako,
Haneji Tatsuji
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00821-6
Subject(s) - immunohistochemistry , submandibular gland , salivary gland , microbiology and biotechnology , staining , parotid gland , antibody , protein subunit , isotype , blot , biology , acid phosphatase , phosphatase , chemistry , pathology , monoclonal antibody , enzyme , biochemistry , endocrinology , immunology , medicine , gene
We have analyzed the distribution of the γ1 isotype of rat protein phosphatase type 1 catalytic subunit in rat salivary glands. Formaldehyde‐fixed paraffin sections were reacted with the PP1γ1 antibody using an immunohistochemical method. Positive staining occurred in striated ducts of parotid gland. However, the staining reaction was less intense in submandibular gland. Proteins were also prepared from rat salivary glands and subjected to SDS‐PAGE, followed by Western blotting analysis with the PP1γ1 antibody. The antibody interacted with protein corresponding to an estimated molecular mass of 36 kDa present in the parotid gland. The staining reaction was considerably weaker with the proteins from submandibular gland.