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Elevated cytosolic free Ca 2+ concentrations and massive Ca 2+ accumulation within vacuoles, in yeast mutant lacking PMR1 , a homolog of Ca 2+ ‐ATPase
Author(s) -
Halachmi D.,
Eilam Y.
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00799-5
Subject(s) - vacuole , cytosol , saccharomyces cerevisiae , mutant , atpase , yeast , golgi apparatus , biochemistry , v atpase , microbiology and biotechnology , biology , chemistry , enzyme , endoplasmic reticulum , cytoplasm , gene
The Ca 2+ ‐ATPase homolog of Saccharomyces cerevisiae, PMR1 , cloned by Rudolph et al. (Cell 58 (1989) 133–145) is required for normal Golgi functions. We have investigated the role of Pmr1‐protein in maintaining homeostasis of cytosolic free Ca 2+ concentration ([Ca 2+ ] i ). It was found that exposure to moderately high Ca 2+ concentrations led to elevated levels of [Ca 2+ ] i in cells of pmr1 null mutant, in comparison with cells of pmr2 isogenic mutant (defective in cell‐membrane Na + ‐ATPase) and of an isogenic wild type. In addition, we showed that PMR1 deletion causes massive accumulation of Ca 2+ in the vacuoles and affects the rates of Ca 2+ influx and efflux.