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Cloning and characterization of a bifunctional metabotropic receptor activated by both extracellular calcium and glutamate
Author(s) -
Kubokawa Kaoru,
Miyashita Tomoyuki,
Nagasawa Hiromichi,
Kubo Yoshihiro
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00786-7
Subject(s) - metabotropic glutamate receptor , metabotropic glutamate receptor 1 , metabotropic glutamate receptor 5 , metabotropic glutamate receptor 2 , metabotropic glutamate receptor 7 , metabotropic glutamate receptor 6 , chemistry , metabotropic receptor , biochemistry , glutamate receptor , receptor , microbiology and biotechnology , biology
A cDNA from salmon brain which induces a consistent activation of Ca 2+ dependent Cl − (Ca 2+ −Cl − ) current in Xenopus oocytes in the saline solution was isolated by an expression cloning method. The primary structure showed a high homology with the metabotropic glutamate receptors (mGluR), and a limited homology with the Ca 2+ /polyvalent cation receptors (CaR). The encoded protein was functionally characterized for the sensitivity to extracellular Ca 2+ (Ca o 2+ ), and the consistent activation of Ca 2+ −Cl − current was determined to be due to Ca 2+ contained in the saline solution. It was also confirmed that the encoded protein has a function to sense glutamate. An increase in inositol 1,4,5‐triphosphate turnover was observed when either Ca 2+ or glutamate was applied. Thus, the encoded protein was identified as a bifunctional metabotropic receptor for both Ca 2+ and glutamate. In addition, rat mGluR1α was also shown to be activated by Ca o 2+ . This is the first report of a G‐protein coupled receptor which is activated by two totally different physiological agonists.