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Immunolocalization and pharmacological relevance of oligopeptide transporter PepT1 in intestinal absorption of β‐lactam antibiotics
Author(s) -
Sai Yoshimichi,
Tamai Ikumi,
Sumikawa Hiromi,
Hayashi Kiyomi,
Nakanishi Takeo,
Amano Osamu,
Numata Masayuki,
Iseki Shoichi,
Tsuji Akira
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00778-8
Subject(s) - oligopeptide , transporter , antibiotics , absorption (acoustics) , chemistry , lactam , microbiology and biotechnology , pharmacology , biochemistry , biology , peptide , stereochemistry , gene , physics , acoustics
A polyclonal antibody (anti‐PepT1/C) was raised against the rabbit intestinal H + ‐coupled oligopeptide transporter, PepT1. Anti‐PepT1/C detected 70–80‐kDa protein in crude membranes obtained from rabbit duodenum, jejunum and ileum. PepT1 was localized in the brush‐border of the absorptive epithelial cells by subcellular fractionation of membranes on a sucrose density gradient and by immunohistochemistry using light and electron microscopy. Transport activity for cephalosporins and dipeptide expressed in Xenopus laevis oocytes injected with total mRNA obtained from rabbit small intestine was eliminated completely by prehybridization of the mRNA with antisense oligonucleotide against the 5′‐coding region of rabbit PepT1 cDNA.