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Reduction of 14–16 kDa allergenic proteins in transgenic rice plants by antisense gene
Author(s) -
Tada Yuichi,
Nakase Masayuki,
Adachi Takahiro,
Nakamura Ryo,
Shimada Hiroaki,
Takahashi Masayoshi,
Fujimura Tatsuhito,
Matsuda Tsukasa
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00773-9
Subject(s) - genetically modified rice , gene , transgene , antisense rna , biology , genetically modified crops , microbiology and biotechnology , allergen , electroporation , gene expression , rna , promoter , genetics , allergy , immunology
An antisense gene strategy was applied to suppress the 14–16 kDa allergen gene expression in maturing rice seeds. Gene constructs producing antisense RNAs of the 16 kDa allergen under the control of some rice seed‐specific promoters were introduced into rice by electroporation. Immunoblot and RNA blot analyses of the seeds from the transgenic rice plants using the allergen‐specific monoclonal antibody and a sequence‐specific antisense RNA probe demonstrated that the 14–16 kDa allergen proteins and their transcripts of the seeds from several transgenic lines were present in much lower in amounts than those of the seeds from parental wild‐type rice. The high levels of reduction observed were stably inherited in at least three generations.