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The structure of the lantibiotic lacticin 481 produced by Lactococcus lactis : location of the thioether bridges
Author(s) -
van den Hooven Henno W.,
Lagerwerf Fija M.,
Heerma Wigger,
Haverkamp Johan,
Piard Jean-Christophe,
Hilbers Cornelis W.,
Siezen Roland J.,
Kuipers Oscar P.,
Rollema Harry S.
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00771-5
Subject(s) - lantibiotics , lanthionine , thioether , lactococcus lactis , bacteriocin , chemistry , peptide , stereochemistry , dipeptidase , nisin , biochemistry , biology , bacteria , organic chemistry , enzyme , genetics , antimicrobial , lactic acid
The lantibiotic lacticin 481 is a bacteriocin produced by Lactococcus lactis ssp. lactis . This polypeptide contains 27 amino acids, including the unusual residues dehydrobutyrine and the thioether‐bridging lanthionine and 3‐methyllanthionine. Lacticin 481 belongs to a structurally distinct group of lantibiotics, which also include streptococcin A‐FF22, salivaricin A and variacin. Here we report the first complete structure of this type of lantibiotic. The exact location of the thioether bridges in lacticin 481 was determined by a combination of peptide chemistry, mass spectrometry and NMR spectroscopy, showing connections between residues 9 and 14, 11 and 25, and 18 and 26.