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Phosphatidylcholine transfer protein from bovine liver contains highly unsaturated phosphatidylcholine species
Author(s) -
Geijtenbeek Teunis B.H.,
Westerman Jan,
Heerma Wigger,
Wirtz Karel W.A.
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00770-3
Subject(s) - phosphatidylcholine , chemistry , fast atom bombardment , molecular mass , mass spectrometry , biochemistry , metabolism , composition (language) , phospholipid , chromatography , enzyme , linguistics , philosophy , membrane
The phosphatidylcholine transfer protein (PC‐TP) from bovine liver contains one molecule of non‐covalently bound PC. In order to gain more insight into the physiological function of PC‐TP, PC was extracted from bovine liver PC‐TP and its molecular species composition identified by fast atom bombardment mass spectrometry. The prevailing molecular species were C18:0/C18:1‐, C18:0/C18:2‐, C18:OIC20:4‐, C18:0120:5‐ and C18:OIC22:5‐PC accounting for 85% of the PC species present. This molecular species composition is not representative for what is present in bovine liver where these species account for 43% of the total PC content [Montfoort et al. (1971) Biochim. Biophys. Acta 231, 335–342]. Another striking observation is that PC species carrying a palmitoyl chain at the sn ‐1 position are nearly absent, despite these species being abundantly present in bovine liver. This study suggests that PC‐TP could play a role in the metabolism of highly unsaturated, stearoyl‐containing PC species.