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Activation of platelet‐derived growth factor (PDGF) receptor dephosphorylation in intact Swiss 3T3 cells by elevators of intracellular Ca 2+ and cAMP
Author(s) -
Böhmer Frank-D.,
Böhmer S.-Annette
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00740-5
Subject(s) - dephosphorylation , platelet derived growth factor receptor , forskolin , growth factor receptor , receptor , chemistry , microbiology and biotechnology , growth factor , endocrinology , medicine , phosphorylation , biology , biochemistry , phosphatase
We investigated the modulation of platelet‐derived growth factor (PDGF) receptor dephosphorylation in Swiss 3T3 cells using a novel assay permitting monitoring of receptor dephosphorylation in intact cells. PDGF treatment of the cells reduced the receptor dephospphorylation rate to 41%, the elevators of intracellular Ca 2+ , A23187 and thapsigargin increasing it to 227 and 138%, respectively. The cAMP elevators forskolin and isobutylmethylxanthine also accelerated PDGF receptor dephosphorylation. The involvement of Ca 2+ ‐ and cAMP‐dependent protein kinases in the regulation of PDGF receptor dephosphorylation is suggested.