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Effects of sequence contexts on misincorporation of nucleotides opposite 2‐hydroxyadenine
Author(s) -
Kamiya Hiroyuki,
Kasai Hiroshi
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00714-4
Subject(s) - klenow fragment , oligonucleotide , nucleotide , dna polymerase , dna , polymerase , base pair , dna polymerase i , sequence (biology) , base (topology) , microbiology and biotechnology , nucleic acid sequence , chemistry , biology , stereochemistry , polymerase chain reaction , biochemistry , exonuclease , gene , reverse transcriptase , mathematical analysis , mathematics
Twelve oligonucleotides containing 2‐hydroxyadenine (2‐OH‐Ade) with different neighboring bases were used as templates in DNA polymerase reactions, and the effects of the sequence contexts were investigated. DNA polymerases α and β inserted dTMP and dCMP opposite 2‐OH‐Ade in most of the oligonucleotides tested. The Klenow fragment of DNA polymerase I primarily incorporated dTMP and dGMP. Effects of the 5′‐flanking base of 2‐OH‐Ade was found when the 3′‐flanking base of 2‐OH‐Ade was A or C. Incorporation of dAMP occurred when the oxidized base was located in a 5′ ‐TA ∗ A‐ 3′ (A ∗ represents 2‐OH‐Ade) sequence. These results suggest that the formation of 2‐OH‐Ade in DNA may induce all the mutations involving A (A → G transition, and A → T and A → C transversions) in cells.