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A model phage display subtraction method with potential for analysis of differential gene expression
Author(s) -
Stausbøl-Grøn Brian,
Wind Troels,
Kjær Svend,
Kahns Liselotte,
Hansen Nils J.V.,
Kristensen Peter,
Clark Brian F.C.
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00703-x
Subject(s) - phage display , suppression subtractive hybridization , subtractive color , selection (genetic algorithm) , antibody , subtraction , biology , microbiology and biotechnology , computational biology , gene , genetics , gene expression , computer science , mathematics , physics , artificial intelligence , arithmetic , cdna library , optics
In order to establish a subtractive procedure that makes it possible to enrich selectively phage displayed antibodies directed against proteins constituting a difference between two populations of cells, a competitive selection strategy utilising two solid phases was developed and tested. Antibodies recognising a defined difference between two otherwise identical protein mixtures were isolated and their specificity confirmed. To test further the efficacy of selection inhibition during the competitive selections, selections towards a total cell extract were performed with and without competition from the same extract. An analysis of the resulting phage antibodies confirmed the subtractive nature of the system described.