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Involvement of a single‐stranded DNA binding protein, ssCRE‐BP/Purα, in morphine dependence
Author(s) -
Osugi Takeshi,
Ding Yun,
Tanaka Hidekazu,
Kuo Che-Hui,
Do Eunju,
Irie Yasuyuki,
Miki Naomasa
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00696-5
Subject(s) - morphine , chemistry , dna , microbiology and biotechnology , biology , biochemistry , pharmacology
We have purified a nuclear protein from mouse cerebella that binds to single‐stranded oligo‐DNA of cAMP response element and is modulated by morphine treatment. Isolation of the cDNA clone showed that the nuclear protein (ssCRE‐BP) was identical to Purα, a DNA binding protein for single‐stranded purine‐rich sequences that was originally isolated as a replication factor. ssCRE‐BP/Purα and mRNA were abundant in the brain. The levels of ssCRE‐BP/Purα and the transcript were not changed by chronic morphine treatment, however, the levels of an activator of ssCRE‐BP/Purα, which is necessary for the DNA binding, may be modulated by the treatment.