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Immunohistochemical localisation of the serotonin 5‐HT2B receptor in mouse gut, cardiovascular system, and brain
Author(s) -
Choi Doo-Sup,
Maroteaux Luc
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00695-3
Subject(s) - polyclonal antibodies , antiserum , immunohistochemistry , receptor , serotonin , biology , microbiology and biotechnology , 5 ht receptor , pathology , endocrinology , antibody , immunology , biochemistry , medicine
We recently reported the cloning of a new member of the serotonin 5‐HT2 family, the 5‐HT2B receptor. We now report the production and characterisation of a specific antiserum directed against the C‐terminal portion of the mouse 5‐HT2B receptor. After affinity purification, this polyclonal antibody recognises specifically the mouse 5‐HT2B receptor. Immunohistochemical analysis of cryosections from various adult mouse tissues reveals a major 5‐HT2B receptor expression in stomach, intestine and pulmonary smooth muscles as well as in myocardium. Furthermore, the antiserum recognises specific areas of the mouse brain, including cerebellar Purkinje cells and their projection areas.