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A novel murine cathelin‐like protein expressed in bone marrow
Author(s) -
Popsueva Anna E.,
Zinovjeva Marina V.,
Visser Jan W.M.,
Zijlmans J.Mark J.M.,
Fibbe Willem E.,
Belyavsky Alexander V.
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00692-8
Subject(s) - peptide , complementary dna , bone marrow , microbiology and biotechnology , antimicrobial , biology , antimicrobial peptides , homologous chromosome , recombinant dna , chemistry , gene , biochemistry , immunology
A novel cDNA encoding a putative secreted protein was isolated from murine bone marrow. The encoded protein named MCLP ( urine athelin‐ ike rotein) was found to be highly homologous to the pig cathelin, and to four neutrophil antimicrobial polypeptides: CAP 18, indolicidin, Bac 5 and FALL‐39. Secondary structure prediction studies identified a highly cationic region in the C‐terminal part of prepro‐MCLP with a tendency to adopt an amphipathic α‐helical conformation, as observed in many antimicrobial peptides. However, no antibacterial activity was observed with the synthetic peptide corresponding to this region of MCLP.