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Crystallization and preliminary X‐ray analysis of human brain hexokinase
Author(s) -
Aleshin Alexander E.,
Zeng Chenbo,
Fromm Herbert J.,
Honzatko Richard B.
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00688-6
Subject(s) - crystallization , hexokinase , polyethylene glycol , solvent , crystallography , crystal (programming language) , chemistry , molecule , resolution (logic) , volume (thermodynamics) , materials science , analytical chemistry (journal) , biochemistry , chromatography , enzyme , organic chemistry , glycolysis , physics , artificial intelligence , computer science , programming language , quantum mechanics
Human brain hexokinase type I, expressed in Escherichia coli , has been crystallized from polyethylene glycol 8000 in the presence of inorganic phosphate. The crystals are hexagonal needles of diameter 0.25 mm, diffracting to a resolution of 3.5 Å on a rotating‐anode/area‐detector system. The crystals belong to the space group P3 1 21/P3 2 21 with cell dimensions . The specific volume of the crystal is 4.2 Å 3 /Da, suggesting an asymmetric unit with a single 100 kDa molecule and a solvent content of 71% by volume or two molecules of hexokinase with a solvent content of 41%. The complex of hexokinase with glucose crystallizes under similar conditions, giving crystals of the same morphology.