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Surface display on staphylococci: a comparative study
Author(s) -
Robert Alain,
Samuelson Patrik,
Andréoni Christine,
Bächi Thomas,
Uhlén Mathias,
Binz Hans,
Ståhl Stefan
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00684-9
Subject(s) - staphylococcus xylosus , receptor , glycoprotein , biology , heterologous , cell sorting , cell , microbiology and biotechnology , chemistry , bacteria , gene , staphylococcus , staphylococcus aureus , biochemistry , genetics
Two different host‐vector expression systems, designed for cell surface display of heterologous receptors on Staphylococcus xylosus and Staphylococcus carnosus , respectively, were compared for the surface display of four variants of a 101 amino acid region derived from the G glycoprotein of human respiratory syncytial virus (RSV). Surface localization of the different chimeric receptors was evaluated by a colorimetric assay and by fluorescence‐activated cell sorting. It was concluded that the S. carnosus system was better both in the ability to translocate inefficiently secreted peptides and in the number of exposed hybrid receptors. The potential use of the described staphylococci as live bacterial vaccine vehicles or alternatives to filamentous phages for surface display of protein libraries is discussed.