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Sudden depletion of carbon source blocks translation, but not transcription, in the yeast Saccharomyces cerevisiae
Author(s) -
Martínez-Pastor M.T.,
Estruch F.
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00683-7
Subject(s) - derepression , saccharomyces cerevisiae , yeast , transcription (linguistics) , invertase , carbon source , reporter gene , lac operon , chemistry , gene expression , gene , biochemistry , biology , sucrose , psychological repression , linguistics , philosophy
Expression of invertase in the yeast Saccharomyces cerevisiae is greatly delayed when derepression occurs in a medium that lacks a usable carbon source. The delay is not a consequence of defects in the transcription of the SUC2 gene but is due to the impossibility of translating the normal levels of mRNA generated under derepressing conditions. The inhibition of translation in the absence of glucose has to be considered when reporter genes such as E. coli lacZ are used to measure transcription in conditions of carbon source starvation.