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Fusion of reconstituted influenza virus envelopes with liposomes mediated by streptavidin/biotin interactions
Author(s) -
Schoen Pieter,
Leserman Lee,
Wilschut Jan
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00682-5
Subject(s) - biotinylation , liposome , lipid bilayer fusion , endosome , hemagglutinin (influenza) , streptavidin , biotin , endocytosis , ganglioside , sialic acid , chemistry , biochemistry , endocytic cycle , viral envelope , vesicle , virus , glycoprotein , virology , biology , membrane , receptor
Reconstituted influenza virus envelopes (virosomes) containing the viral hemagglutinin (HA) represent an efficient fusogenic cellular delivery system. By interaction of HA with its natural receptors, sialylated lipids (gangliosides) or proteins, virosomes bind to cells and, following endocytic uptake, deliver their contents to the cytosol through fusion from within acidic endosomes. Here, we show that binding to sialic acid is not necessary for fusion. In the presence of streptavidin, virosomes containing a biotinylated lipid fused with liposomes lacking sialic acid if these liposomes also had a biotinylated lipid in their membranes. Moreover, fusion characteristics corresponded well with fusion of virosomes with ganglioside‐containing liposomes.