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Identification and characterisation of a homologue of p64 in rat tissues
Author(s) -
Howell Steven,
Duncan Rory R.,
Ashley Richard H.
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00676-x
Subject(s) - polyclonal antibodies , microbiology and biotechnology , western blot , fusion protein , biology , polyacrylamide gel electrophoresis , gel electrophoresis , biochemistry , gene , kidney , microsome , antibody , genetics , recombinant dna , enzyme
Previous work has suggested that the gene encoding p64, a component of a bovine kidney intracellular chloride channel, may be a member of a gene family. We have raised a polyclonal antibody to an E. coli fusion protein which has sequence similarity to p64. Immunoblotting detected a protein in rat brain, kidney, liver and lung. In rat brain, the protein was enriched in cerebellar microsomal membranes. Western blot analyses of denaturing and blue native polyacrylamide gels indicated that the protein is a single non‐disulphide‐linked polypeptide chain with an apparent M r of 43 kDa that contributes to a native protein complex with an apparent M r of 130 kDa.