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Fructose 2,6‐bisphosphate induces irreversible transitions in cell‐free extracts of rat liver
Author(s) -
Frenzel Jochen,
Schellenberger Wolfgang,
Eschrich Klaus
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00664-3
Subject(s) - fructose 2,6 bisphosphate , bistability , allosteric regulation , fructose , fructose 1,6 bisphosphatase , chemistry , biophysics , phosphofructokinase , glycolysis , 2 acetylaminofluorene , enzyme , biochemistry , biology , physics , quantum mechanics , microsome
The effect of fructose 2,6‐bisphosphate on the dynamics of the 6‐phosphofructo‐1‐kinase/fructose‐1,6‐bisphos‐phatase cycle is investigated in a cell‐free extract of rat liver under steady‐state conditions. Bistability emerges on the basis of the reciprocal allosteric modulation of 6‐phosphofructo‐1‐kinase and fructose‐1,6‐bisphosphatase. Under conditions of bistability fructose 2,6‐bisphosphate may cause transitions between alternative steady states. However, in contrast to what is frequently observed in bistable systems, within a broad range of experimental conditions these transitions proceed irreversibly from states with high ATP to states characterized by low ATP.