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Gluconeogenesis from ascorbic acid: ascorbate recycling in isolated murine hepatocytes
Author(s) -
Braun László,
Puskás Ferenc,
Csala Miklós,
Gyórffy Erika,
Garzó Tamás,
Mandl József,
Bánhegyi Gábor
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00654-0
Subject(s) - pentose phosphate pathway , gluconeogenesis , ascorbic acid , biochemistry , chemistry , dehydroascorbic acid , pentose , stimulation , glycolysis , metabolism , vitamin c , biology , food science , endocrinology , fermentation
Ascorbic acid synthesis and breakdown were investigated in isolated hepatocytes prepared from fasted mice. Stimulation of gluconeogenesis by alanine or xylitol led to ascorbate synthesis. On the other hand, ascorbate or dehydroascorbate addition resulted in concentration‐dependent glucose production and elevation of the pentose phosphate pathway intermediate xylulose 5‐phosphate. Stimulation of ascorbate oxidation and/or the inhibition of dehydroascorbate reduction increased glucose formation. Inhibition of the pentose phosphate pathway decreased glucose production from dehydroascorbate with increased accumulation of xylulose 5‐phosphate. These results suggest that ascorbate can be recycled by a novel way involving intermediates of the pentose phosphate pathway, gluconeogenesis and hexuronic acid pathway.