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Determination of relative abundance of splicing variants of Oreochromis glutamate receptors by quantitative reverse‐transcriptase PCR
Author(s) -
Wu Yi-Mi,
Kung Shieh-Shiuh,
Chow Wei-Yuan
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00649-7
Subject(s) - reverse transcriptase , biology , rna splicing , real time polymerase chain reaction , glutamate receptor , microbiology and biotechnology , receptor , messenger rna , gene , polymerase chain reaction , genetics , rna
In this study, the relative abundance of splicing variants of Oreochromis non‐NMDA subtype glutamate receptors was studied by quantitative reverse‐transcriptase PCR (RT‐PCR). The relative expression level between the flip and flop transcripts of fGluR2α determined by quantitative RT‐PCR is apparently much higher than that estimated by sequence analysis of the cloned RT‐PCR products. Control studies were performed to demonstrate the accuracy of the application of quantitative RT‐PCR analysis in studying the relative abundance between the flip and flop transcripts of glutamate receptors.