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An electrophysiological study of calcium entry during normal human T‐lymphocyte activation
Author(s) -
Ricci Riccardo,
Buffelli Mario,
Riviera Anna P.,
Cangiano Alberto
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00630-8
Subject(s) - jurkat cells , stimulation , calcium , depolarization , electrophysiology , t lymphocyte , chemistry , t cell , cell culture , cell , microbiology and biotechnology , biophysics , lymphocyte , immunology , neuroscience , biology , in vitro , biochemistry , immune system , genetics , organic chemistry
Our aim was to observe whether normal human T‐cells respond to mitogenic stimulation with large whole‐cell inward currents (composed of identifiable single‐channel contributions) when [Ca 2+ ] i is not markedly lowered but instead kept normal or moderately low, as has been reported in human leukaemic Jurkat T‐cell line and T‐cell clones [Kuno et al. (1986) Nature 323, 269–273; Kuno and Gardner (1987) Nature 326, 301–304; Gardner (1990) Annu. Rev. Immunol. 8, 231–252]. Whole‐cell patch recordings showed no such currents in cells otherwise normally responding to depolarisation with the macroscopic I K described in T‐lymphocytes and thus deemed viable, in agreement with the notion that Ca 2+ influx in normal T‐cells enterily depends on depletion of internal stores [Putney (1986) Cell Calcium 7, 1–12; Putney (1990) Cell Calcium 11, 611–624].