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The construction and characterization of an effective transpositional system based on IS30
Author(s) -
Farkas Tibor,
Kiss János,
Olasz Ferenc
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00626-6
Subject(s) - computational biology , characterization (materials science) , chemistry , computer science , biochemical engineering , biology , engineering , nanotechnology , materials science
We constructed an in vivo system to detect transpositional rearrangements induced by the insertion sequence IS30. The transposase protein expressed from the transposase producer plasmids catalyzed rearrangements on different target sequences presented in trans . High yields, up to 83%, of transpositional frequencies were observed. The frequency of rearrangements correlated with the amount of transposase protein produced and the attractivity of the target sequences. Alteration in the frequency of transposition was observed in the recA − E. coli strains JM109 and TG2. Remarkable structural and functional analogy was found with site‐specific recombination systems.