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Identification of DNA binding‐site preferences for nuclear factor I‐A
Author(s) -
Osada Shigehiro,
Daimon Shoko,
Nishihara Tsutomu,
Imagawa Masayoshi
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00622-9
Subject(s) - dna binding site , binding site , dna binding protein , biology , dna , promoter , genetics , hmg box , transcription factor , dna replication , microbiology and biotechnology , computational biology , gene , gene expression
Nuclear factor I (NFI) proteins constitute a large family of DNA binding proteins. These proteins promote the initiation of adenovirus replication and regulate the transcription of viral and cellular genes. The binding sites for NFI have been reported in a wide variety of promoters, and they exhibit flexibility in their sequences. To clarify the DNA binding site of NFI‐A, one of the NFI proteins, we performed a polymerase chain reaction‐mediated random site selection, and determined the optimal sequence as 5′‐TTGGCANNNN(G/T)CCA(G/A)‐3′.