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The secG deletion mutation of Escherichia coli is suppressed by expression of a novel regulatory gene of Bacillus subtilis
Author(s) -
Kontinen Vesa P.,
Helander Ilkka M.,
Tokuda Hajime
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00602-3
Subject(s) - bacillus subtilis , escherichia coli , gene , mutation , genetics , chemistry , gene expression , biology , microbiology and biotechnology , bacteria
SecG, a membrane component of E. coli protein translocase, stimulates the translocation of proteins across the cell membrane through the cycle of topology inversion, which is coupled to the membrane‐insertion and deinsertion cycle of SecA [Nishiyama et al. (1996) Cell 85, 71–81]. A gene of B. subtilis able to suppress the cold‐sensitive phenotype of the secG deletion mutant of E. coli was cloned and found to encode a novel regulatory protein, ScgR. Similarity search revealed homology with known proteins such as GlnR of B. subtilis . Plasmid‐encoded ScgR stimulated protein translocation in the deletion mutant. ScgR increased the proportion of cardiolipin at the expense of phosphatidylglycerol, but did not affect the composition of other lipid components of the cell, suggesting that the increased cardiolipin level compensates for the SecG function and thereby stimulates protein translocation.