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Fish glucocorticoid receptor with splicing variants in the DNA binding domain
Author(s) -
Takeo Jiro,
Hata Jun-ichiro,
Segawa Chisako,
Toyohara Haruhiko,
Yamashita Shinya
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00596-0
Subject(s) - glucocorticoid receptor , dna , fish <actinopterygii> , rna splicing , alternative splicing , chemistry , glucocorticoid , computational biology , genetics , microbiology and biotechnology , biology , receptor , gene , fishery , exon , endocrinology , rna
Here we describe the isolation of a rainbow trout cDNA containing an entire GR coding region. Although the encoded protein is highly homologous to other GRs, especially in its DNA binding domain, it contains a nine amino acid insertion between the two zinc fingers. This novel form is found in all rainbow trout tissues examined; however, the testis also contains a splice variant lacking this insert, making it completely continuous to other GRs. In transient transfection assays of cultured cells, the two rainbow trout GR variants activated transcription from the glucocorticoid‐responsive mouse mammary tumor virus promoter to comparable levels.