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Nitric oxide oxidises a ferrous mammalian lipoxygenase to a pre‐activated ferric species
Author(s) -
Wiesner Rainer,
Rathmann Jörg,
Holzhütter Hermann-Georg,
Stöβer Reinhard,
Mäder Karsten,
Nolting Hans,
Kühn Hartmut
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00591-1
Subject(s) - ferrous , nitric oxide , chemistry , lipoxygenase , ferric , hydrogen peroxide , enzyme , biochemistry , oxide , inorganic chemistry , organic chemistry
Nitric oxide is known as an inhibitor of soybean lipoxygenase‐1. Investigating the interaction of a mammalian 15‐lipoxygenase with nitric oxide, we found that this enzyme is also inhibited reversibly when incubated with nitric oxide for a short time period (5 s) under anaerobic conditions. This inhibition may be due to the formation of a dissociable lipoxygenase‐nitric oxide complex. With longer incubation periods the ferrous lipoxygenase is oxidised to a ferric form. This oxidation renders the enzyme more susceptible to peroxide activation, causing a time‐dependent shortening of the kinetic lag phase.