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In vivo gene electroinjection and expression in rat liver
Author(s) -
Heller Richard,
Jaroszeski Mark,
Atkin Andrew,
Moradpour Darius,
Gilbert Richard,
Wands Jack,
Nicolau Claude
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00590-x
Subject(s) - electroporation , in vivo , transfection , microbiology and biotechnology , biology , luciferase , gene expression , gene , gene delivery , biochemistry , genetics
In vivo targeted gene transfer by non‐viral vectors is subjected to anatomical constraints depending on the route of administration. Transfection efficiency and gene expression in vivo using non‐viral vectors is also relatively low. We report that in vivo electropermeabilization of the liver tissue of rats in the presence of genes encoding luciferase or β‐galactosidase resulted in the strong expression of these genetic markers in rat liver cells. About 30–40% of the rat liver cells electroporated expressed the β‐galactosidase genetic marker 48 h after electroporation. The marker expression was also detected at least 21 days after transfection at about 5% of the level 48 h after electroporation. The results indicate that gene transfer by electroporation in vivo may avoid anatomical constraints and low transfection efficiency.