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Maltokinase (ATP: maltose 1‐phosphotransferase) from Actinoplanes sp.: demonstration of enzyme activity and characterization of the reaction product
Author(s) -
Drepper Anne,
Peitzmann Reinhard,
Pape Hermann
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00554-6
Subject(s) - maltose , phosphotransferase , biochemistry , chemistry , enzyme , acarbose , nuclear magnetic resonance spectroscopy , phosphate , stereochemistry
Cell free extract of the acarbose producer Actinoplanes sp. catalyzes ATP‐dependent phosphorylation of maltose. This was shown by two different assays. The product was purified and its structure determined to be α‐maltose‐1‐phosphate by chemical analysis and NMR spectroscopy.