Premium
Glutathione depletion induces glycogenolysis dependent ascorbate synthesis in isolated murine hepatocytes
Author(s) -
Braun László,
Csala Miklós,
Poussu Anssi,
Garzó Tamás,
Mandl József,
Bánhegyi Gábor
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00548-0
Subject(s) - glycogenolysis , glutathione , dithiothreitol , chemistry , menadione , biochemistry , glycogen , ascorbic acid , medicine , endocrinology , glycogen synthase , biology , oxidative stress , enzyme , food science
The relationship between glutathione deficiency, glycogen metabolism and ascorbate synthesis was investigated in isolated murine hepatocytes. Glutathione deficiency caused by various agents increased ascorbate synthesis with a stimulation of glycogen breakdown. Increased ascorbate synthesis from UDP‐glucose or gulonolactone could not be further affected by glutathione depletion. Fructose prevented the stimulated glycogenolysis and ascorbate synthesis caused by glutathione consumption. Reduction of oxidised glutathione by dithiothreitol decreased the elevated glycogenolysis and ascorbate synthesis in diamide or menadione treated hepatocytes. Our results suggest that a change in GSH/GSSG ratio seems to be a sufficient precondition of altering glycogenolysis and a consequent ascorbate synthesis.