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Leukotriene A 4 , and not leukotriene B 4 , is the main 5‐lipoxygenase metabolite released by bovine leukocytes
Author(s) -
Sala Angelo,
Testa Tullio,
Folco Giancarlo
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00539-x
Subject(s) - metabolite , leukotriene b4 , chemistry , leukotriene , lipoxygenase , ionophore , arachidonate 5 lipoxygenase , platelet , bovine serum albumin , biochemistry , transcellular , enzyme , arachidonic acid , immunology , biology , membrane , inflammation , asthma
The production of leukotriene A 4 (LTA 4 )‐derived metabolites, analysed by RP‐HPLC, was studied in purified bovine polymorphonuclear leukocyte (PMNL) preparations and in PMNL‐platelet coincubations after challenge with the calcium ionophore A23187. The results obtained show that in bovine PMNL LTB 4 represents the main LTA 4 metabolite. When washed platelets were added to PMNL, LTC 4 was the main enzymatic metabolite observed, indicating a substantial transfer of PMNL‐derived LTA 4 to platelets. The synthesis of LTC 4 was accompanied by a significant decrease in LTB 4 , suggesting that a quota of the LTB 4 synthesized in PMNL preparations is the result of transcellular metabolism of released LTA 4 by neighbouring PMNL. Reduction of PMNL‐PMNL interactions through dilution of cell incubates allowed us to estimate that most of the leukotriene A 4 synthesized by PMNL is indeed released from the cell. LTA 4 , and not LTB 4 , represents the main 5‐lipoxygenase metabolite released by bovine PMNL.