Premium
Upregulation of PKC δ‐ and downregulation of PKC α‐mRNA and protein by a phorbol ester in human T84 cells
Author(s) -
Assert R.,
Schatz H.,
Pfeiffer A.
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00522-4
Subject(s) - protein kinase c , downregulation and upregulation , messenger rna , phorbol , microbiology and biotechnology , chemistry , second messenger system , signal transduction , biology , biochemistry , gene
Protein kinase C (PKC) family members are downregulated by chronic activation at the protein level in most cell types. In T84 human epithelial cells 12‐ O ‐tetradecanoyl phorbol 13‐acetate (TPA) caused persistent translocation of PKC δ to the membrane compartment and a 400% increase of PKC δ‐mRNA after 24 h. In contrast, PKC α protein was completely downregulated and its mRNA was decreased to 60% of control levels after 24 h. This is the first report of PKC δ‐mRNA upregulation by TPA which was previously only shown for PKCβ. In view of the antimitogenic actions of PKC δ this pattern of regulation may serve to preserve growth control even in the presence of chronic cell activation.