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Characterization of glucose transport in the hyperthermophilic Archaeon Sulfolobus solfataricus
Author(s) -
Cusdin Fiona S.,
Robinson Matthew J.,
Holman Geoffrey D.,
Hough David W.,
Danson Michael J.
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00497-8
Subject(s) - sulfolobus solfataricus , sulfolobus , chemistry , archaea , biochemistry , gene
Sulfolobus solfataricus is a hyperthermophilic Archaeon growing at 80°C, pH 3. The glucose transport system of this organism has been characterised kinetically at this temperature and pH using 2‐deoxy‐ d ‐glucose: the sugar analogue is transported into the cells with a K m =1.8±0.3 μ M and a V max =3.6±0.1 nmol min −1 (mg protein) −1 , with an intracellular accumulation of up to 200‐fold over the extracellular concentration. Transport was significantly reduced at pH 5. Inhibition of 2‐deoxy‐ d ‐glucose transport was investigated using a variety of sugars and sugar analogues; d ‐glucose, d ‐galactose and d ‐mannose showed the highest affinity for the transporter, with d ‐glucose possessing a K i =120±20 nM.