Premium
Insulin‐like growth factor‐I (IGF‐I) mRNA in the small intestine of suckling and adult rats
Author(s) -
Dvořák Bohuslav,
Stephana Audrey L.,
Holubec Hana,
Williams Catherine S.,
Philipps Anthony F.,
Koldovskoý Otakar
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00495-4
Subject(s) - messenger rna , small intestine , insulin like growth factor , endocrinology , biology , growth factor , medicine , in situ hybridization , jejunum , reverse transcription polymerase chain reaction , gene expression , insulin , microbiology and biotechnology , gene , receptor , biochemistry
The presence and cellular localization of insulin‐like growth factor‐I (IGF‐I) mRNA in the small intestine of suckling and adult rats was studied. A sensitive reverse transcription (RT) competitive‐polymerase chain reaction (PCR) revealed IGF‐I gene expression in both age groups. Adult tissue contained 3‐fold higher levels of IGF‐I mRNA in comparison with sucklings. Using an in situ hybridization technique, IGF‐I transcripts were localized mainly in enterocytes and goblet cells in the intestinal crypts of adult rats. By using this technique, IGF‐I mRNA was not detected in jejunum of 12‐day‐old rats.