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Selective control of cytosolic glutathione peroxidase and phospholipid hydroperoxide glutathione peroxidase mRNA stability by selenium supply
Author(s) -
Bermano Giovanna,
Arthur John R.,
Hesketh John E.
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00493-0
Subject(s) - phospholipid hydroperoxide glutathione peroxidase , glutathione peroxidase , gpx4 , peroxidase , selenium , messenger rna , gpx1 , gpx3 , polysome , biochemistry , chemistry , gpx6 , phospholipid , cytosol , puromycin , glutathione , microbiology and biotechnology , enzyme , biology , protein biosynthesis , ribosome , rna , gene , organic chemistry , membrane
Selenium depletion of H4 hepatoma cells reduced cytosolic glutathione peroxidase (cGSH‐Px) mRNA abundance but had no effect on phospholipid hydroperoxide glutathione peroxidase (PHGSH‐Px) mRNA abundance. Actinomycin D chase experiments showed that selenium depletion had no effect on the stability of PHGSH‐Px mRNA but decreased the stability of cGSH‐Px mRNA. In Se‐replete cells puromycin decreased the stability of both cGSH‐Px and PHGSH‐Px mRNAs. The results suggest that when selenium supply is limiting PHGSH‐Px mRNA translation is maintained more than that of cGSH‐Px mRNA, and thus more cGSH‐Px mRNA is released from polysomes and degraded.