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Drosophila melanogaster SL2 cells contain a hypoxically inducible DNA binding complex which recognises mammalian HIF‐1 binding sites
Author(s) -
Nagao Masaya,
Ebert Benjamin L.,
Ratcliffe Peter J.,
Pugh Christopher W.
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00484-x
Subject(s) - cycloheximide , drosophila melanogaster , biology , microbiology and biotechnology , phosphoglycerate kinase , dna , biochemistry , gene , protein biosynthesis
Nuclear extracts from Drosophila SL2 cells were found to contain a hypoxically inducible complex capable of binding to hypoxia response elements from mammalian genes. This complex (HIF‐D) resembled mammalian hypoxia inducible factor (HIF‐1) in DNA sequence specificity, abrogation of induction by cycloheximide, induction by desferrioxamine and redox sensitivity of DNA binding. However, HIF‐D was not induced by cobalt and was less sensitive to phosphatase than HIF‐1. Endogenous phosphoglycerate kinase mRNA in SL2 cells showed similar inducible characteristics to HIF‐D. These findings are evidence that the mammalian HIF‐1 dependent system of oxygen regulated gene expression has a functional homologue in Drosophila .