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The inhibitor protein of phosphorylated nitrate reductase from spinach ( Spinacia oleracea ) leaves is a 14‐3‐3 protein
Author(s) -
Bachmann Markus,
Huber Joan L.,
Liao Pao-Chi,
Gage Douglas A.,
Huber Steven C.
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00478-4
Subject(s) - spinacia , spinach , nitrate reductase , monoclonal antibody , biochemistry , peptide , chemistry , recombinant dna , peptide sequence , chenopodiaceae , microbiology and biotechnology , enzyme , antibody , biology , gene , chloroplast , immunology
The inhibitor protein (IP) that inactivates spinach leaf NADH:nitrate reductase (NR) has been identified for the first time as a member of the eukaryotic 14‐3‐3 protein family based on three lines of evidence. First, the sequence of an eight amino acid tryptic peptide, obtained from immunopurified IP, matched that of a highly conserved region of the 14‐3‐3 proteins. Second, an authentic member of the 14‐3‐3 family, recombinant Arabidopsis GF14ω, caused inactivation of phospho‐NR in a magnesium‐dependent manner identical to IP. Third, an anti‐GF14 monoclonal antibody cross‐reacted with IP and anti‐IP monoclonal antibodies cross‐reacted with GF14ω.