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Combined effects of ATP and its analogs on the membrane permeability in transformed mouse fibroblasts
Author(s) -
Arav Ruth,
Friedberg Ilan
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00472-3
Subject(s) - extracellular , membrane , biophysics , permeability (electromagnetism) , chemistry , membrane potential , adenosine triphosphate , membrane permeability , cell membrane , cell , biochemistry , microbiology and biotechnology , biology
Extracellular ATP (0.6 mM) induces a marked decrease in the membrane potential, followed by an increase in cell membrane permeability in transformed mouse fibroblasts. The effects of the ATP analogs, p[CH 2 ]ppA and p[NH]ppA (0.6 mM), on the membrane potential and permeability are much less pronounced. ATP at 0.05 mM has no effect by itself, but markedly increases the analog‐induced membrane potential dissipation and permeability. The data suggest that ATP‐induced membrane permeation is composed of two processes: One is common to ATP and its analogs and appears to be a receptormediated process. The second is unique for ATP, effective even at low concentration (0.05 mM), and might be mediated by cell surface enzymes, for which ATP, but not its analogs, serves as a substrate.